Rapid detection of Melissococcus Plotinus the agent of European foulbrood in honey bee colonies by PCR

Abstract

Abstract:
European foul brood, is a honey bee larvae disease. EFB is a dangerous disease of the bee colonies that considered to be caused by Gram-positive Melissococcus plutonius. The disease is widely distributed in the world and has created a growing problem in some areas. Because of recent advances in molecular technology, identification of bacteria is very easy and nucleic acid detection technology is rapidly replacing traditional methods of microbiology. European foul brood, is located in the list of dangerous diseases for the health of animals. The disease is endemic in many parts of areas and provide seasonal epidemic. The scenario of this disease varies in different countries.
In this study with standard strain, PCR was developed by specific primers. PCR products electrophpresed on 1 %  agarose gel.Larval samples using culture and PCR methode, were tested simultaneously.Eightsamples werepositive by PCRand2 sampleswere positiveby culture.

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References

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