Evaluate of the combined effects of bacteria Bacillus thuringiensis and nuclear polyhedra virus (NPV) on third instar larvae of wax moth, (Galleria mellonella L.) in laboratory conditions

Authors

1 Researcher, Agricultural and Natural Resources Research and Education Institute of West Azerbaijan Province, Urmia, Iran.

2 Associated Professor, Department of Animal Science, Faculty of Agricultural Science, Urmia university, Urmia, Iran.

3 Associate professor, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran.

4 Associate professor, Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran.

Abstract

Abstract
The wax moth (Galleria mellonella L.) is one of the most important pests of Apis mellifera beehives. Then, the aim of this study is to use bacteria (Bacillus thuringiensis B.) and nuclear polyhedrosis virus (NPV) main and combined effects on the wax moth third-instar larvae in laboratory conditions. Large wax moth larvae were collected from apiaries and transported to the laboratory and reared to laying. After bioassay and determination of LC50 and LC25 of bacteria and virus, their combined effect on third instar larvae was assessed. In order to determine the lethality of the compounds, the mortality of larval ages modified with the Abbott equation and were analyzed by probit analysis and LC50 values were calculated. Analysis of variance and comparing the means was carried out using SPSS software var. 22. Our results reveal that the highest and the lowest mortality were related to the treatment of the combined biological agents (LC25 NPV+LC25B.t) and the control treatments, respectively. The effect of sublethal dose (LC15) of B. thuringiensis and NPV on the conversion of larval stage to pupal stage and pupal stage to adult insect according to the mean of treatments, the highest conversion was related to the control treatment and the lowest was related to NPV treatment. Our results showed that, although both B. thuringiensis and NPV are effective in controlling the larval stage of the wax moth, the combined form of these two biological agents is more effective in the integrated management of this pest.

Keywords

Honeybee Science Journal
Volume 15, Issue 29
September 2024

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