Isolation and identification of symbiotic Lactobacillus bacteria in the honeybee (Apis Mellifera) gastrointestinal tract

Authors

1 Graduated student (MS), Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran.

2 Associated Professor, Department of Animal Science, Faculty of Agricultural Science, Urmia university, Urmia, Iran.

3 , Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran

4 Department of Honeybee, Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

5 Department of Engineering Research, West Azerbaijan Agricultural and Natural Resources Research and Education Organization (AREEO), Urmia, Iran

Abstract

Abstract
The honeybee is a useful insect with high impact on agricultural products and the ecosystem pollination, then on food security and GDP. Considering that keeping honeybees in nature and helping pollination through honeybees depends solely on the economic efficiency of the honeybee industry and the subsequent desire of beekeepers to raise it. Therefore, increasing the functional production of honeybee colonies, along with maintaining genetic diversity, is the main goals of genetic breeder and policy makers in the honeybee industry. One of the most common and widely used methods to preserve honeybees in nature and the beekeeping industry is to increase honeybee productive and reproductive performances by developing knowledge-based nutritional methods. In this regard, in the present study, isolation and identification of the symbiotic lactic acid bacteria in the honeybee's digestive system was carried out. This information and isolated microbial compounds can be used in the fermentation of complementary food supplements for honeybees to help the digestibility of produced foods for honeybees. The purpose of this study is to isolate beneficial bacteria from the digestive system of honeybees in the regions of West Azerbaijan, East Azerbaijan and Gilan provinces and to investigate the probiotic indicators of the isolates. Here, the sampling and isolation methods of the bacterial isolates from the bee's digestive system were presented, and the isolated bacteria were identified and grouped using catalase tests and gram staining. Finally, the identification of the desired strains by the microscope, maintain and cultivate was provided.

Keywords